› 1z3w

X-ray diffraction
1.7Å resolution

Structure of Phanerochaete chrysosporium cellobiohydrolase Cel7D (CBH58) in complex with cellobioimidazole

Released:
DOI: 10.2210/pdb1z3w/pdb
PDB entry 1z3w coloured by chain, front view.
PDB entry 1z3w coloured by chain, side view.
PDB entry 1z3w coloured by chain, top view.
Source organism: Phanerodontia chrysosporium
Primary publication:
Structures of Phanerochaete chrysosporium Cel7D in complex with product and inhibitors.
Ubhayasekera W, Muñoz IG, Vasella A, Ståhlberg J, Mowbray SL
FEBS J 272 1952-64 (2005)
PMID: 15819888

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 45.78 KDa
Source organism: Phanerodontia chrysosporium
UniProt:
  • Canonical: Q7LIJ0 (Residues: 19-449; Coverage: 88%)
Gene names: CBH, CBHI.2, cbh1-4
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand IDC 1 x IDC
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 87.382Å b: 46.578Å c: 98.505Å
α: 90° β: 102.58° γ: 90°
R-values:
R R work R free
0.181 0.178 0.227

Quick links

Citations

2 review citations

Towards a molecular-level theory of carbohydrate processivity in glycoside hydrolases.
Beckham et al. (2014)
1 more

2 mentions without citation

Structural, biochemical, and computational characterization of the glycoside hydrolase family 7 cellobiohydrolase of the tree-killing fungus Heterobasidion irregulare.
Momeni et al. (2013)
1 more