› 1z25

X-ray diffraction
2.7Å resolution

Structure of P.furiosus Argonaute with bound Mn2+

Released:
DOI: 10.2210/pdb1z25/pdb
PDB entry 1z25 coloured by chain, front view.
PDB entry 1z25 coloured by chain, side view.
PDB entry 1z25 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Purified Argonaute2 and an siRNA form recombinant human RISC.
Rivas FV, Tolia NH, Song JJ, Aragon JP, Liu J, Hannon GJ, Joshua-Tor L
Nat Struct Mol Biol 12 340-9 (2005)
PMID: 15800637

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute Chain: A
Molecule details ›
Chain: A
Length: 771 amino acids
Theoretical weight: 90.6 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3D2 (Residues: 1-770; Coverage: 100%)
Gene names: PF0537, ago
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 68.662Å b: 105.108Å c: 73.662Å
α: 90° β: 102.7° γ: 90°
R-values:
R R work R free
0.26 0.215 0.274
Expression system: Escherichia coli

Quick links

Citations

118 review citations

Origins and Mechanisms of miRNAs and siRNAs.
Carthew et al. (2009)
117 more

8 mentions without citation

Structural and functional modules in RNA interference.
Nowotny et al. (2009)
7 more