› 1yj3

X-ray diffraction
1.6Å resolution

Crystal structure analysis of product bound methionine aminopeptidase Type 1c from Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb1yj3/pdb
PDB entry 1yj3 coloured by chain, front view.
PDB entry 1yj3 coloured by chain, side view.
PDB entry 1yj3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Identification of an SH3-binding motif in a new class of methionine aminopeptidases from Mycobacterium tuberculosis suggests a mode of interaction with the ribosome.
Addlagatta A, Quillin ML, Omotoso O, Liu JO, Matthews BW
Biochemistry 44 7166-74 (2005)
PMID: 15882055

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase 2 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.75 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WK19 (Residues: 1-285; Coverage: 100%)
Gene names: MTV003.07c, Rv2861c, map, mapB
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

4 bound ligands:
Ligand CO 2 x CO
Ligand K 1 x K
Ligand MET 1 x MET
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 49.36Å b: 48.16Å c: 56.54Å
α: 90° β: 95.05° γ: 90°
R-values:
R R work R free
0.233 0.202 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure and function of the molecular chaperone Trigger Factor.
Hoffmann et al. (2010)
7 more

4 mentions without citation

Catalysis and inhibition of Mycobacterium tuberculosis methionine aminopeptidase.
Lu et al. (2010)
3 more