› 1yhb

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURES OF Y41H AND Y41F MUTANTS OF GENE V PROTEIN FROM FF PHAGE SUGGEST POSSIBLE PROTEIN-PROTEIN INTERACTIONS IN GVP-SSDNA COMPLEX

Released:
DOI: 10.2210/pdb1yhb/pdb
PDB entry 1yhb coloured by chain, front view.
PDB entry 1yhb coloured by chain, side view.
PDB entry 1yhb coloured by chain, top view.
Source organism: Enterobacteria phage f1
Primary publication:
Crystal structures of Y41H and Y41F mutants of gene V protein from Ff phage suggest possible protein-protein interactions in the GVP-ssDNA complex.
Guan Y, Zhang H, Konings RN, Hilbers CW, Terwilliger TC, Wang AH
Biochemistry 33 7768-78 (1994)
PMID: 8011642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-Binding protein G5P Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.68 KDa
Source organism: Enterobacteria phage f1
Expression system: Not provided
UniProt:
  • Canonical: P69543 (Residues: 1-87; Coverage: 100%)
Gene name: V
Sequence domains: Helix-destabilising protein
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 74.97Å b: 27.9Å c: 42.55Å
α: 90° β: 103.09° γ: 90°
R-values:
R R work R free
0.18 0.18 not available
Expression system: Not provided

Quick links

Citations

3 review citations

Structure and assembly of filamentous bacteriophages.
Marvin et al. (2014)
2 more

3 mentions without citation

Thoroughly sampling sequence space: large-scale protein design of structural ensembles.
Larson et al. (2002)
2 more