› 1yf5

X-ray diffraction
2.75Å resolution

Cyto-Epsl: The Cytoplasmic Domain Of Epsl, An Inner Membrane Component Of The Type II Secretion System Of Vibrio Cholerae

Released:
DOI: 10.2210/pdb1yf5/pdb
PDB entry 1yf5 coloured by chain, front view.
PDB entry 1yf5 coloured by chain, side view.
PDB entry 1yf5 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae.
Abendroth J, Murphy P, Sandkvist M, Bagdasarian M, Hol WG
J Mol Biol 348 845-55 (2005)
PMID: 15843017

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein L Chain: L
Molecule details ›
Chain: L
Length: 254 amino acids
Theoretical weight: 28.53 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45782 (Residues: 1-248; Coverage: 61%)
Gene names: VC_2725, epsL
Sequence domains: Type II secretion system (T2SS), protein L
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 61.006Å b: 88.707Å c: 55.371Å
α: 90° β: 106.01° γ: 90°
R-values:
R R work R free
0.216 0.213 0.269
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
16 more

2 mentions without citation

Crystal structure of the full-length ATPase GspE from the Vibrio vulnificus type II secretion system in complex with the cytoplasmic domain of GspL.
Lu et al. (2014)
1 more