› 1y7h

X-ray diffraction
2.52Å resolution

Structural and biochemical studies identify tobacco SABP2 as a methylsalicylate esterase and further implicate it in plant innate immunity, Northeast Structural Genomics Target AR2241

Released:
DOI: 10.2210/pdb1y7h/pdb
PDB entry 1y7h coloured by chain, front view.
PDB entry 1y7h coloured by chain, side view.
PDB entry 1y7h coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity.
Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L
Proc Natl Acad Sci U S A 102 1773-8 (2005)
PMID: 15668381

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Salicylic acid-binding protein 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 268 amino acids
Theoretical weight: 30.83 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6RYA0 (Residues: 1-260; Coverage: 100%)
Gene names: LOC107791137, SABP2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SCN 8 x SCN
1 modified residue:
Ligand MSE 72 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 45.63Å b: 137.736Å c: 171.208Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.229 0.227 0.297
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Salicylic Acid, a multifaceted hormone to combat disease.
Vlot et al. (2009)
26 more

4 mentions without citation

Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity.
Forouhar et al. (2005)
3 more