› 1y3h

X-ray diffraction
2.8Å resolution

Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1y3h/pdb
PDB entry 1y3h coloured by chain, front view.
PDB entry 1y3h coloured by chain, side view.
PDB entry 1y3h coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex.
Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K
Biochem Biophys Res Commun 327 500-8 (2005)
PMID: 15629142

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 32.96 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHV7 (Residues: 1-307; Coverage: 100%)
Gene names: MTCI125.17, Rv1695, ppnK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: C2
Unit cell:
a: 140.176Å b: 69.443Å c: 106.433Å
α: 90° β: 130.29° γ: 90°
R-values:
R R work R free
0.235 0.221 0.235
Expression system: Escherichia coli

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Citations

6 review citations

The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.
Pollak et al. (2007)
5 more

1 mention without citation

Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5.
Ando et al. (2011)