› 1xxv

X-ray diffraction
2.5Å resolution

Yersinia YopH (residues 163-468) binds phosphonodifluoromethyl-Phe containing hexapeptide at two sites

Released:
DOI: 10.2210/pdb1xxv/pdb
PDB entry 1xxv coloured by chain, front view.
PDB entry 1xxv coloured by chain, side view.
PDB entry 1xxv coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
Two substrate-targeting sites in the Yersinia protein tyrosine phosphatase co-operate to promote bacterial virulence.
Ivanov MI, Stuckey JA, Schubert HL, Saper MA, Bliska JB
Mol Microbiol 55 1346-56 (2005)
PMID: 15720545

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147289 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase YopH Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 33.52 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15273 (Residues: 163-468; Coverage: 65%)
Gene names: yop51, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Epidermal growth factor receptor derived peptide Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 8 amino acids
Theoretical weight: 863 Da
Source organism: Yersinia enterocolitica
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FTY 4 x FTY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 54.13Å b: 47.17Å c: 71.82Å
α: 104.45° β: 115.05° γ: 89.97°
R-values:
R R work R free
0.178 0.178 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

Yersinia outer proteins: role in modulation of host cell signaling responses and pathogenesis.
Viboud et al. (2005)
10 more

1 mention without citation

Druggability analysis and classification of protein tyrosine phosphatase active sites.
Ghattas et al. (2016)