› 1xvm

X-ray diffraction
1.1Å resolution

Trypsin from Fusarium oxysporum- room temperature to atomic resolution

Released:
DOI: 10.2210/pdb1xvm/pdb
PDB entry 1xvm coloured by chain, front view.
PDB entry 1xvm coloured by chain, side view.
PDB entry 1xvm coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Extraction of functional motion in trypsin crystal structures.
Schmidt A, Lamzin VS
Acta Crystallogr D Biol Crystallogr 61 1132-9 (2005)
PMID: 16041079

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152785 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 22.2 KDa
Source organism: Fusarium oxysporum
UniProt:
  • Canonical: P35049 (Residues: 25-248; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
substrate tripeptide GLY-ALA-ARG Chain: B
Molecule details ›
Chain: B
Length: 3 amino acids
Theoretical weight: 303 Da
Source organism: Fusarium oxysporum
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P1
Unit cell:
a: 33.277Å b: 37.037Å c: 40.1Å
α: 102.22° β: 103.93° γ: 102.59°
R-values:
R R work R free
0.144 0.144 not available
Expression system: Not provided

Quick links

Citations

5 citation in other articles

Molecular dynamics simulation of triclinic lysozyme in a crystal lattice.
Janowski et al. (2016)
4 more

1 mention without citation

Characterization of three novel genetic loci encoding bacteriocins associated with Xanthomonas perforans.
Marutani-Hert et al. (2020)