› 1xqs

X-ray diffraction
2.9Å resolution

Crystal structure of the HspBP1 core domain complexed with the fragment of Hsp70 ATPase domain

Released:
DOI: 10.2210/pdb1xqs/pdb
PDB entry 1xqs coloured by chain, front view.
PDB entry 1xqs coloured by chain, side view.
PDB entry 1xqs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of Hsp70 function by HspBP1: structural analysis reveals an alternate mechanism for Hsp70 nucleotide exchange.
Shomura Y, Dragovic Z, Chang HC, Tzvetkov N, Young JC, Brodsky JL, Guerriero V, Hartl FU, Bracher A
Mol Cell 17 367-79 (2005)
PMID: 15694338

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143651 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hsp70-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 31.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZL4 (Residues: 84-359; Coverage: 77%)
Gene names: HSPBP, HSPBP1, PP1845
Sequence domains: Nucleotide exchange factor Fes1
Structure domains: Leucine-rich Repeat Variant
Heat shock 70 kDa protein 1A Chains: C, D
Molecule details ›
Chains: C, D
Length: 191 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV8 (Residues: 184-371; Coverage: 29%)
Gene names: HSP72, HSPA1, HSPA1A, HSX70
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 73.484Å b: 94.809Å c: 155.704Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.296
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Molecular chaperones and protein quality control.
Bukau et al. (2006)
29 more

18 mentions without citation

The nucleotide exchange factors of Hsp70 molecular chaperones.
Bracher et al. (2015)
17 more