› 1xml

X-ray diffraction
2Å resolution

Structure of human Dcps

Released:
DOI: 10.2210/pdb1xml/pdb
PDB entry 1xml coloured by chain, front view.
PDB entry 1xml coloured by chain, side view.
PDB entry 1xml coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping.
Chen N, Walsh MA, Liu Y, Parker R, Song H
J Mol Biol 347 707-18 (2005)
PMID: 15769464

Function and Biology Details

Reaction catalysed: 
A 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
m7GpppX diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 39.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96C86 (Residues: 1-337; Coverage: 100%)
Gene names: DCPS, DCS1, HINT5, HSPC015
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 101.922Å b: 103.863Å c: 71.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Early nonsense: mRNA decay solves a translational problem.
Amrani et al. (2006)
4 more

4 mentions without citation

Sequence and structural analyses of nuclear export signals in the NESdb database.
Xu et al. (2012)
3 more