› 1xex

X-ray diffraction
2.5Å resolution

Structural biochemistry of ATP-driven dimerization and DNA stimulated activation of SMC ATPases.

Released:
DOI: 10.2210/pdb1xex/pdb
PDB entry 1xex coloured by chain, front view.
PDB entry 1xex coloured by chain, side view.
PDB entry 1xex coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural biochemistry of ATP-driven dimerization and DNA-stimulated activation of SMC ATPases.
Lammens A, Schele A, Hopfner KP
Curr Biol 14 1778-82 (2004)
PMID: 15458651

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162541 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromosome partition protein Smc Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.23 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZY2 (Residues: 1-182; Coverage: 16%)
Gene names: PF1843, smc
Structure domains: P-loop containing nucleotide triphosphate hydrolases
SMC protein Chain: B
Molecule details ›
Chain: B
Length: 172 amino acids
Theoretical weight: 19.18 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 102.6Å b: 102.6Å c: 87.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.261
Expression system: Escherichia coli

Quick links

Citations

28 review citations

KATP channels as molecular sensors of cellular metabolism.
Nichols CG. (2006)
27 more

9 mentions without citation

Crystal structure of Bacillus stearothermophilus UvrA provides insight into ATP-modulated dimerization, UvrB interaction, and DNA binding.
Pakotiprapha et al. (2008)
8 more