› 1xbn

X-ray diffraction
2.5Å resolution

Crystal structure of a bacterial nitric oxide sensor: an ortholog of mammalian soluble guanylate cyclase heme domain

Released:
DOI: 10.2210/pdb1xbn/pdb
PDB entry 1xbn coloured by chain, front view.
PDB entry 1xbn coloured by chain, side view.
PDB entry 1xbn coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
Femtomolar sensitivity of a NO sensor from Clostridium botulinum.
Nioche P, Berka V, Vipond J, Minton N, Tsai AL, Raman CS
Science 306 1550-3 (2004)
PMID: 15472039

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.86 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-191; Coverage: 32%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:
Ligand OXY 1 x OXY
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P4232
Unit cell:
a: 120.607Å b: 120.607Å c: 120.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.263 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

NO-independent stimulators and activators of soluble guanylate cyclase: discovery and therapeutic potential.
Evgenov et al. (2006)
26 more

4 mentions without citation

How do heme-protein sensors exclude oxygen? Lessons learned from cytochrome c', Nostoc puntiforme heme nitric oxide/oxygen-binding domain, and soluble guanylyl cyclase.
Tsai et al. (2012)
3 more