› 1xae

X-ray diffraction
2.7Å resolution

Crystal structure of wild type yellow fluorescent protein zFP538 from Zoanthus

Released:
DOI: 10.2210/pdb1xae/pdb
PDB entry 1xae coloured by chain, front view.
PDB entry 1xae coloured by chain, side view.
PDB entry 1xae coloured by chain, top view.
Source organism: Zoanthus sp.
Primary publication:
zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore.
Remington SJ, Wachter RM, Yarbrough DK, Branchaud B, Anderson DC, Kallio K, Lukyanov KA
Biochemistry 44 202-12 (2005)
PMID: 15628861

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein FP538 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.2 KDa
Source organism: Zoanthus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y4 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand BME 2 x BME
3 modified residues:
Ligand CSO 4 x CSO
Ligand NFA 2 x NFA
Ligand CH7 2 x CH7

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 121.16Å b: 121.16Å c: 111.69Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.255
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
22 more

2 mentions without citation

Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
Stepanenko et al. (2008)
1 more