› 1x2i

X-ray diffraction
1.45Å resolution

Crystal Structure Of Archaeal Xpf/Mus81 Homolog, Hef From Pyrococcus Furiosus, Helix-hairpin-helix Domain

Released:
DOI: 10.2210/pdb1x2i/pdb
PDB entry 1x2i coloured by chain, front view.
PDB entry 1x2i coloured by chain, side view.
PDB entry 1x2i coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural and functional analyses of an archaeal XPF/Rad1/Mus81 nuclease: asymmetric DNA binding and cleavage mechanisms.
Nishino T, Komori K, Ishino Y, Morikawa K
Structure 13 1183-92 (2005)
PMID: 16084390

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 75 amino acids
Theoretical weight: 8.49 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZH8 (Residues: 691-764; Coverage: 10%)
Gene name: PF2015
Sequence domains: Helix-hairpin-helix motif
Structure domains: 5' to 3' exonuclease, C-terminal subdomain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU, SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 39.942Å b: 56.889Å c: 68.371Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.238
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structural and functional relationships of the XPF/MUS81 family of proteins.
Ciccia et al. (2008)
8 more

4 mentions without citation

All DACs in a Row: Domain Architectures of Bacterial and Archaeal Diadenylate Cyclases.
Galperin MY. (2023)
3 more