› 1x1w

X-ray diffraction
2.1Å resolution

Water-mediate interaction at aprotein-protein interface

Released:
DOI: 10.2210/pdb1x1w/pdb
PDB entry 1x1w coloured by chain, front view.
PDB entry 1x1w coloured by chain, side view.
PDB entry 1x1w coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Water-mediated interaction at a protein-protein interface
Ikura T, Urakubo Y, Ito N
Chem Phys 307 111-119 (2004)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132803 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases
Barstar Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 90 amino acids
Theoretical weight: 10.23 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11540 (Residues: 1-90; Coverage: 100%)
Sequence domains: Barstar (barnase inhibitor)
Structure domains: Barstar-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 200.807Å b: 44.04Å c: 83.748Å
α: 90° β: 110.36° γ: 90°
R-values:
R R work R free
0.219 0.219 0.265
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

Influence of Grid Spacing in Poisson-Boltzmann Equation Binding Energy Estimation.
Harris et al. (2013)
1 more