› 1wxc

X-ray diffraction
1.2Å resolution

Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein

Released:
DOI: 10.2210/pdb1wxc/pdb
PDB entry 1wxc coloured by chain, front view.
PDB entry 1wxc coloured by chain, side view.
PDB entry 1wxc coloured by chain, top view.
Source organism: Streptomyces castaneoglobisporus
Primary publication:
Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis.
Matoba Y, Kumagai T, Yamamoto A, Yoshitsu H, Sugiyama M
J Biol Chem 281 8981-90 (2006)
PMID: 16436386

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosinase copper-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 32.09 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WS2 (Residues: 1-273; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase
MelC Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.2 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WS1 (Residues: 1-126; Coverage: 100%)
Sequence domains: Tyrosinase co-factor MelC1
Structure domains: protein ne1242 domain like

Ligands and Environments

1 bound ligand:
Ligand NO3 4 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 64.664Å b: 96.734Å c: 54.574Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.208
Expression system: Escherichia coli

Quick links

Citations

43 review citations

An updated review of tyrosinase inhibitors.
Chang TS. (2009)
42 more

9 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
8 more