› 1wrj

X-ray diffraction
2Å resolution

Crystal structure of O6-methylguanine methyltransferase from Sulfolobus tokodaii

Released:
DOI: 10.2210/pdb1wrj/pdb
PDB entry 1wrj coloured by chain, front view.
PDB entry 1wrj coloured by chain, side view.
PDB entry 1wrj coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii
Entry authors: Kuroda M, Tsunoda M, Nakamura KT

Function and Biology Details

Reaction catalysed: 
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylated-DNA--protein-cysteine methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.98 KDa
Source organism: Sulfurisphaera tokodaii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q973C7 (Residues: 1-156; Coverage: 100%)
Gene names: STK_09670, ogt
Sequence domains: 6-O-methylguanine DNA methyltransferase, DNA binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 48.32Å b: 51.62Å c: 62.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.219
Expression system: Escherichia coli

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