› 1wox

X-ray diffraction
2.1Å resolution

Crystal structure of heme oxygenase-2 from Synechocystis sp. PCC 6803 in complex with heme and NO

Released:
DOI: 10.2210/pdb1wox/pdb
PDB entry 1wox coloured by chain, front view.
PDB entry 1wox coloured by chain, side view.
PDB entry 1wox coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Crystal structure of dimeric heme oxygenase-2 from Synechocystis sp. PCC 6803 in complex with heme.
Sugishima M, Hagiwara Y, Zhang X, Yoshida T, Migita CT, Fukuyama K
Biochemistry 44 4257-66 (2005)
PMID: 15766254

Function and Biology Details

Reaction catalysed: 
Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme oxygenase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 28.57 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P74133 (Residues: 1-250; Coverage: 100%)
Gene names: pbsA2, sll1875
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand NO 2 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 57.31Å b: 73.76Å c: 71.07Å
α: 90° β: 106.91° γ: 90°
R-values:
R R work R free
0.213 0.211 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Heme oxygenase and heme degradation.
Kikuchi et al. (2005)
11 more