› 1w0m

X-ray diffraction
2.5Å resolution

Triosephosphate isomerase from Thermoproteus tenax

Released:
DOI: 10.2210/pdb1w0m/pdb
PDB entry 1w0m coloured by chain, front view.
PDB entry 1w0m coloured by chain, side view.
PDB entry 1w0m coloured by chain, top view.
Source organism: Thermoproteus tenax
Primary publication:
Structure and function of a regulated archaeal triosephosphate isomerase adapted to high temperature.
Walden H, Taylor GL, Lorentzen E, Pohl E, Lilie H, Schramm A, Knura T, Stubbe K, Tjaden B, Hensel R
J Mol Biol 342 861-75 (2004)
PMID: 15342242

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 226 amino acids
Theoretical weight: 23.31 KDa
Source organism: Thermoproteus tenax
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NKN9 (Residues: 1-226; Coverage: 100%)
Gene names: TTX_0494, tpi, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 8 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P6522
Unit cell:
a: 186.893Å b: 186.893Å c: 287.757Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.226
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Unusual pathways and enzymes of central carbohydrate metabolism in Archaea.
Siebers et al. (2005)
2 more

3 mentions without citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
2 more