› 1vzo

X-ray diffraction
1.8Å resolution

The structure of the N-terminal kinase domain of MSK1 reveals a novel autoinhibitory conformation for a dual kinase protein

Released:
DOI: 10.2210/pdb1vzo/pdb
PDB entry 1vzo coloured by chain, front view.
PDB entry 1vzo coloured by chain, side view.
PDB entry 1vzo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of MSK1 reveals a novel autoinhibitory conformation for a dual kinase protein.
Smith KJ, Carter PS, Bridges A, Horrocks P, Lewis C, Pettman G, Clarke A, Brown M, Hughes J, Wilkinson M, Bax B, Reith A
Structure 12 1067-77 (2004)
PMID: 15274926

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal protein S6 kinase alpha-5 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 39.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75582 (Residues: 2-348; Coverage: 43%)
Gene names: MSK1, RPS6KA5
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BME 1 x BME
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 66.629Å b: 73.772Å c: 89.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.242
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
4 more

10 mentions without citation

Dynamics-Driven Allostery in Protein Kinases.
Kornev et al. (2015)
9 more