› 1vhh

X-ray diffraction
1.7Å resolution

A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG

Released:
DOI: 10.2210/pdb1vhh/pdb
PDB entry 1vhh coloured by chain, front view.
PDB entry 1vhh coloured by chain, side view.
PDB entry 1vhh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A potential catalytic site revealed by the 1.7-A crystal structure of the amino-terminal signalling domain of Sonic hedgehog.
Hall TM, Porter JA, Beachy PA, Leahy DJ
Nature 378 212-6 (1995)
PMID: 7477329

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sonic hedgehog protein N-product Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62226 (Residues: 34-195; Coverage: 39%)
Gene names: Hhg1, Shh
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 53.7Å b: 79Å c: 35.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.261
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Communicating with Hedgehogs.
Hooper et al. (2005)
30 more

24 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
23 more