› 1va4

X-ray diffraction
1.8Å resolution

Pseudomonas fluorescens aryl esterase

Released:
DOI: 10.2210/pdb1va4/pdb
PDB entry 1va4 coloured by chain, front view.
PDB entry 1va4 coloured by chain, side view.
PDB entry 1va4 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Structure of an aryl esterase from Pseudomonas fluorescens.
Cheeseman JD, Tocilj A, Park S, Schrag JD, Kazlauskas RJ
Acta Crystallogr D Biol Crystallogr 60 1237-43 (2004)
PMID: 15213385

Function and Biology Details

Reaction catalysed: 
A phenyl acetate + H(2)O = a phenol + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arylesterase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 279 amino acids
Theoretical weight: 30.97 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22862 (Residues: 2-272; Coverage: 100%)
Gene name: estF
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand GOL 20 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P32
Unit cell:
a: 146.04Å b: 146.04Å c: 129.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.175 0.202
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Enzymes from Extreme Environments and Their Industrial Applications.
Littlechild JA. (2015)
3 more

16 mentions without citation

Switching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase.
Yin et al. (2010)
15 more