› 1v2x

X-ray diffraction
1.5Å resolution

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanosine(18) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(18) in tRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanosine(18)-2'-O)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 22.12 KDa
Source organism: Thermus thermophilus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5SM16 (Residues: 1-194; Coverage: 100%)
Gene names: TTHA0127, trmH
Sequence domains:
Structure domains: Alpha/beta knot

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 77.601Å b: 44.023Å c: 56.756Å
α: 90° β: 120.78° γ: 90°
R-values:
R R work R free
0.229 0.225 0.277
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Structural and sequence motifs of protein (histone) methylation enzymes.
Cheng et al. (2005)
14 more

13 mentions without citation

Transfer RNA methyltransferases with a SpoU-TrmD  (SPOUT) fold and their modified nucleosides in  tRNA.
Hori H. (2017)
12 more