› 1v2d

X-ray diffraction
1.9Å resolution

Crystal Structure of T.th HB8 Glutamine Aminotransferase

Released:
DOI: 10.2210/pdb1v2d/pdb
PDB entry 1v2d coloured by chain, front view.
PDB entry 1v2d coloured by chain, side view.
PDB entry 1v2d coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of glutamine:phenylpyruvate aminotransferase from Thermus thermophilus HB8: induced fit and substrate recognition.
Goto M, Omi R, Miyahara I, Hosono A, Mizuguchi H, Hayashi H, Kagamiyama H, Hirotsu K
J Biol Chem 279 16518-25 (2004)
PMID: 14761974

Function and Biology Details

Reaction catalysed: 
L-glutamine + pyruvate = 2-oxoglutaramate + L-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 42.32 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WK2 (Residues: 1-381; Coverage: 100%)
Gene name: TthHB5018_10190
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2221
Unit cell:
a: 77.8Å b: 91.9Å c: 110.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.218
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han et al. (2010)
2 more

4 mentions without citation

Structural Consideration of the Working Mechanism of Fold Type I Transaminases From Eubacteria: Overt and Covert Movement.
Kwon et al. (2019)
3 more