› 1v0m

X-ray diffraction
1.07Å resolution

Xylanase Xyn10a from Streptomyces lividans in complex with xylobio-deoxynojirimycin at pH 7.5

Released:
DOI: 10.2210/pdb1v0m/pdb
PDB entry 1v0m coloured by chain, front view.
PDB entry 1v0m coloured by chain, side view.
PDB entry 1v0m coloured by chain, top view.
Source organism: Streptomyces lividans
Primary publication:
Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.
Gloster TM, Williams SJ, Roberts S, Tarling CA, Wicki J, Withers SG, Davies GJ
Chem Commun (Camb) 1794-5 (2004)
PMID: 15306887

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 34.13 KDa
Source organism: Streptomyces lividans
Expression system: Streptomyces lividans
UniProt:
  • Canonical: P26514 (Residues: 42-354; Coverage: 72%)
Gene name: xlnA
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand XDN 1 x XDN
Ligand XYP 1 x XYP
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 66.884Å b: 46.081Å c: 85.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.099 0.098 0.121
Expression system: Streptomyces lividans

Quick links

Citations

1 review citation

High-resolution crystallography and drug design.
Cachau et al. (2005)
6 other articles

1 mention without citation

Internal motion in protein crystal structures.
Schmidt et al. (2010)