› 1ux8

X-ray diffraction
2.15Å resolution

X-ray structure of truncated oxygen-avid haemoglobin from Bacillus subtilis

Released:
DOI: 10.2210/pdb1ux8/pdb
PDB entry 1ux8 coloured by chain, front view.
PDB entry 1ux8 coloured by chain, side view.
PDB entry 1ux8 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The truncated oxygen-avid hemoglobin from Bacillus subtilis: X-ray structure and ligand binding properties.
Giangiacomo L, Ilari A, Boffi A, Morea V, Chiancone E
J Biol Chem 280 9192-202 (2005)
PMID: 15590662

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 2 truncated hemoglobin YjbI Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.14 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31607 (Residues: 1-132; Coverage: 100%)
Gene names: BSU11560, yjbI
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand CYN 1 x CYN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P41212
Unit cell:
a: 41.193Å b: 41.193Å c: 134.537Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Structure and regulation of soluble guanylate cyclase.
Derbyshire et al. (2012)
8 more

6 mentions without citation

Evolutionary and Functional Relationships in the Truncated Hemoglobin Family.
Bustamante et al. (2016)
5 more