› 1uws

X-ray diffraction
1.95Å resolution

Structure of beta-glycosidase from Sulfolobus solfataricus in complex with 2-deoxy-2-fluoro-glucose

Released:
DOI: 10.2210/pdb1uws/pdb
PDB entry 1uws coloured by chain, front view.
PDB entry 1uws coloured by chain, side view.
PDB entry 1uws coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors.
Gloster TM, Roberts S, Ducros VM, Perugino G, Rossi M, Hoos R, Moracci M, Vasella A, Davies GJ
Biochemistry 43 6101-9 (2004)
PMID: 15147194

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 56.76 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22498 (Residues: 1-489; Coverage: 100%)
Gene names: SSO3019, lacS
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand G2F 2 x G2F
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 167.605Å b: 167.605Å c: 95.448Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.229
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Synthesis of novel bioactive lactose-derived oligosaccharides by microbial glycoside hydrolases.
Díez-Municio et al. (2014)
1 more

1 mention without citation

Biochemical and structural characterization of a thermostable β-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.
Hassan et al. (2015)