› 1uun

X-ray diffraction
2.5Å resolution

Main porin from Mycobacterium smegmatis (MspA)

Released:
DOI: 10.2210/pdb1uun/pdb
PDB entry 1uun coloured by chain, front view.
PDB entry 1uun coloured by chain, side view.
PDB entry 1uun coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
The structure of a mycobacterial outer-membrane channel.
Faller M, Niederweis M, Schulz GE
Science 303 1189-92 (2004)
PMID: 14976314

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin MspA Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 19.5 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QR29 (Residues: 28-211; Coverage: 100%)
Gene names: MSMEG_0965, MSMEI_0939, mspA
Sequence domains: MspA
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I422
Unit cell:
a: 113.86Å b: 113.86Å c: 229.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.249
Expression system: Escherichia coli

Quick links

Citations

37 review citations

The bacterial cell envelope.
Silhavy et al. (2010)
36 more

30 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
29 more