› 1usz

X-ray diffraction
3.28Å resolution

SeMet AfaE-3 adhesin from Escherichia Coli

Released:
DOI: 10.2210/pdb1usz/pdb
PDB entry 1usz coloured by chain, front view.
PDB entry 1usz coloured by chain, side view.
PDB entry 1usz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
High resolution studies of the Afa/Dr adhesin DraE and its interaction with chloramphenicol.
Pettigrew D, Anderson KL, Billington J, Cota E, Simpson P, Urvil P, Rabuzin F, Roversi P, Nowicki B, du Merle L, Le Bouguénec C, Matthews S, Lea SM
J Biol Chem 279 46851-7 (2004)
PMID: 15331605

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Afimbrial adhesin AFA-III Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57254 (Residues: 20-160; Coverage: 100%)
Gene names: afaE-3, afaE3
Sequence domains: Dr-family adhesin
Structure domains: Dr adhesin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I432
Unit cell:
a: 141.3Å b: 141.3Å c: 141.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.247
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek.
Nuccio et al. (2007)
13 more