› 1ul3

X-ray diffraction
2Å resolution

Crystal Structure of PII from Synechocystis sp. PCC 6803

Released:
DOI: 10.2210/pdb1ul3/pdb
PDB entry 1ul3 coloured by chain, front view.
PDB entry 1ul3 coloured by chain, side view.
PDB entry 1ul3 coloured by chain, top view.
Source organism: Synechocystis sp.
Primary publication:
The structures of the PII proteins from the cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803.
Xu Y, Carr PD, Clancy P, Garcia-Dominguez M, Forchhammer K, Florencio F, Vasudevan SG, Tandeau de Marsac N, Ollis DL
Acta Crystallogr D Biol Crystallogr 59 2183-90 (2003)
PMID: 14646076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 112 amino acids
Theoretical weight: 12.42 KDa
Source organism: Synechocystis sp.
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q55247 (Residues: 1-112; Coverage: 100%)
Gene names: glnB, ssl0707
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 129.569Å b: 129.569Å c: 74.329Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.219 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Nitrogen regulation in bacteria and archaea.
Leigh et al. (2007)
6 more

2 mentions without citation

The 2.2 A resolution crystal structure of Bacillus cereus Nif3-family protein YqfO reveals a conserved dimetal-binding motif and a regulatory domain.
Godsey et al. (2007)
1 more