› 1uj8

X-ray diffraction
1.75Å resolution

Structures of ORF3 in Two Crystal Forms, a Member of Isc Machinery of E. coli Involved in the Assembly of Iron-Sulfur Clusters

Released:
DOI: 10.2210/pdb1uj8/pdb
PDB entry 1uj8 coloured by chain, front view.
PDB entry 1uj8 coloured by chain, side view.
PDB entry 1uj8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli YfhJ protein, a member of the ISC machinery involved in assembly of iron-sulfur clusters.
Shimomura Y, Takahashi Y, Kakuta Y, Fukuyama K
Proteins 60 566-9 (2005)
PMID: 15937904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein IscX Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0L9 (Residues: 2-66; Coverage: 99%)
Gene names: JW2508, b2524, iscX, yfhJ
Sequence domains: Iron-sulphur cluster assembly
Structure domains: IscX-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 76.63Å b: 27.25Å c: 39.68Å
α: 90° β: 111° γ: 90°
R-values:
R R work R free
0.194 0.194 0.231
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular paleontology and complexity in the last eukaryotic common ancestor.
Koumandou et al. (2013)
2 more

4 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
3 more