› 1uj6

X-ray diffraction
1.74Å resolution

Crystal structure of Thermus thermophilus ribose-5-phosphate isomerase complexed with arabinose-5-phosphate

Released:
DOI: 10.2210/pdb1uj6/pdb
PDB entry 1uj6 coloured by chain, front view.
PDB entry 1uj6 coloured by chain, side view.
PDB entry 1uj6 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Oxyanion hole-stabilized stereospecific isomerization in ribose-5-phosphate isomerase (Rpi).
Hamada K, Ago H, Sugahara M, Nodake Y, Kuramitsu S, Miyano M
J Biol Chem 278 49183-90 (2003)
PMID: 13679361

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-180858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase A Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 24.28 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72J47 (Residues: 1-227; Coverage: 100%)
Gene names: TT_C0932, rpiA
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand A5P 1 x A5P
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: C2221
Unit cell:
a: 62.349Å b: 62.541Å c: 131.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Triosephosphate isomerase: a highly evolved biocatalyst.
Wierenga et al. (2010)
1 more

2 mentions without citation

CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii.
Lykins et al. (2018)
1 more