› 1ucd

X-ray diffraction
1.3Å resolution

Crystal structure of Ribonuclease MC1 from bitter gourd seeds complexed with 5'-UMP

Released:
DOI: 10.2210/pdb1ucd/pdb
PDB entry 1ucd coloured by chain, front view.
PDB entry 1ucd coloured by chain, side view.
PDB entry 1ucd coloured by chain, top view.
Source organism: Momordica charantia
Entry authors: Suzuki A, Numata T, Yao M, Kimura M, Tanaka I

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease MC Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.23 KDa
Source organism: Momordica charantia
UniProt:
  • Canonical: P23540 (Residues: 1-191; Coverage: 100%)
Gene name: MC1
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

2 bound ligands:
Ligand U5P 1 x U5P
Ligand URA 1 x URA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 39.18Å b: 63.48Å c: 73.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.203

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