› 1u5t

X-ray diffraction
3.6Å resolution

Structure of the ESCRT-II endosomal trafficking complex

Released:
DOI: 10.2210/pdb1u5t/pdb
PDB entry 1u5t coloured by chain, front view.
PDB entry 1u5t coloured by chain, side view.
PDB entry 1u5t coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the ESCRT-II endosomal trafficking complex.
Hierro A, Sun J, Rusnak AS, Kim J, Prag G, Emr SD, Hurley JH
Nature 431 221-5 (2004)
PMID: 15329733

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155605 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vacuolar-sorting protein SNF8 Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12483 (Residues: 1-233; Coverage: 100%)
Gene names: SNF8, VPS22, YPL002C
Sequence domains: EAP30/Vps36 family
Structure domains:
Vacuolar protein-sorting-associated protein 36 Chain: B
Molecule details ›
Chain: B
Length: 169 amino acids
Theoretical weight: 19.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q06696 (Residues: 396-564; Coverage: 30%)
Gene names: GRD12, VAC3, VPL11, VPS36, YLR417W
Sequence domains: EAP30/Vps36 family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Vacuolar protein-sorting-associated protein 25 Chains: C, D
Molecule details ›
Chains: C, D
Length: 202 amino acids
Theoretical weight: 23.58 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P47142 (Residues: 1-202; Coverage: 100%)
Gene names: J1957, VPS25, VPT25, YJR102C
Sequence domains: ESCRT-II complex subunit
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4322
Unit cell:
a: 150.04Å b: 150.04Å c: 185.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.226 0.288
Expression system: Escherichia coli BL21

Quick links

Citations

40 review citations

The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins.
Raiborg et al. (2009)
39 more

6 mentions without citation

Reverse-topology membrane scission by the ESCRT proteins.
Schöneberg et al. (2017)
5 more