› 1u5p

X-ray diffraction
2Å resolution

Crystal Structure of Repeats 15 and 16 of Chicken Brain Alpha Spectrin

Released:
DOI: 10.2210/pdb1u5p/pdb
PDB entry 1u5p coloured by chain, front view.
PDB entry 1u5p coloured by chain, side view.
PDB entry 1u5p coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Independent movement, dimerization and stability of tandem repeats of chicken brain alpha-spectrin.
Kusunoki H, Minasov G, Macdonald RI, Mondragón A
J Mol Biol 344 495-511 (2004)
PMID: 15522301

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.86 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07751 (Residues: 1662-1876; Coverage: 9%)
Gene names: SPTA2, SPTAN1
Sequence domains: Spectrin repeat
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P4332
Unit cell:
a: 148.2Å b: 148.2Å c: 148.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Spectrins: a structural platform for stabilization and activation of membrane channels, receptors and transporters.
Machnicka et al. (2014)
5 more

16 mentions without citation

What lessons can be learned from studying the folding of homologous proteins?
Nickson et al. (2010)
15 more