› 1u4h

X-ray diffraction
2.07Å resolution

Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (oxygen complex)

Released:
DOI: 10.2210/pdb1u4h/pdb
PDB entry 1u4h coloured by chain, front view.
PDB entry 1u4h coloured by chain, side view.
PDB entry 1u4h coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases.
Pellicena P, Karow DS, Boon EM, Marletta MA, Kuriyan J
Proc Natl Acad Sci U S A 101 12854-9 (2004)
PMID: 15326296

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 22.38 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

4 bound ligands:
Ligand HEM 2 x HEM
Ligand OXY 2 x OXY
Ligand GOL 4 x GOL
Ligand SCN 1 x SCN
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 80.119Å b: 128.951Å c: 43.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.255
Expression system: Escherichia coli

Quick links

Citations

40 review citations

NO-independent stimulators and activators of soluble guanylate cyclase: discovery and therapeutic potential.
Evgenov et al. (2006)
39 more

8 mentions without citation

How do heme-protein sensors exclude oxygen? Lessons learned from cytochrome c', Nostoc puntiforme heme nitric oxide/oxygen-binding domain, and soluble guanylyl cyclase.
Tsai et al. (2012)
7 more