› 1u0u

X-ray diffraction
2.11Å resolution

An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure

Released:
DOI: 10.2210/pdb1u0u/pdb
PDB entry 1u0u coloured by chain, front view.
PDB entry 1u0u coloured by chain, side view.
PDB entry 1u0u coloured by chain, top view.
Source organism: Pinus sylvestris
Primary publication:
An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases.
Austin MB, Bowman ME, Ferrer JL, Schröder J, Noel JP
Chem Biol 11 1179-94 (2004)
PMID: 15380179

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + cinnamoyl-CoA = 4 CoA + pinosylvin + 4 CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pinosylvin synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 397 amino acids
Theoretical weight: 43.12 KDa
Source organism: Pinus sylvestris
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q02323 (Residues: 1-393; Coverage: 100%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 57.221Å b: 361.291Å c: 57.317Å
α: 90° β: 98.39° γ: 90°
R-values:
R R work R free
0.228 0.225 0.289
Expression system: Escherichia coli BL21

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Citations

35 review citations

Resveratrol: its biologic targets and functional activity.
Pervaiz et al. (2009)
34 more

7 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
6 more