› 1tu2

Solution NMR

THE COMPLEX OF NOSTOC CYTOCHROME F AND PLASTOCYANIN DETERMIN WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES

Released:
DOI: 10.2210/pdb1tu2/pdb
PDB entry 1tu2 coloured by chain, ensemble of 10 models, front view.
PDB entry 1tu2 coloured by chain, ensemble of 10 models, side view.
PDB entry 1tu2 coloured by chain, ensemble of 10 models, top view.
Source organism: Nostoc sp. PCC 7119
Primary publication:
Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. PCC 7119 as determined by paramagnetic NMR. The balance between electrostatic and hydrophobic interactions within the transient complex determines the relative orientation of the two proteins.
Díaz-Moreno I, Díaz-Quintana A, De la Rosa MA, Ubbink M
J Biol Chem 280 18908-15 (2005)
PMID: 15705583

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155474 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plastocyanin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.12 KDa
Source organism: Nostoc sp. PCC 7119
Expression system: Escherichia coli
UniProt:
  • Canonical: P46444 (Residues: 35-139; Coverage: 100%)
Gene names: all0258, petE
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome f Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.25 KDa
Source organism: Nostoc sp. PCC 7119
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93SW9 (Residues: 45-298; Coverage: 88%)
Gene names: all2452, petA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand HEC 1 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DYNAMICS
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Dynamics in electron transfer protein complexes.
Bashir et al. (2011)
9 more

3 mentions without citation

The complex of cytochrome f and plastocyanin from Nostoc sp. PCC 7119 is highly dynamic.
Scanu et al. (2012)
2 more