Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132736 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
10 kDa anti-sigma factor
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.6 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
Sequence domains: Anti-Sigma Factor A
Structure domains: Anti-Sigma Factor A
Length: 90 amino acids
Theoretical weight: 10.6 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
- Canonical:
P32267 (Residues: 1-90; Coverage: 100%)
Sequence domains: Anti-Sigma Factor A
Structure domains: Anti-Sigma Factor A
RNA polymerase sigma factor RpoD
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 9.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Length: 81 amino acids
Theoretical weight: 9.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00579 (Residues: 533-613; Coverage: 13%)
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
dynamical simulated annealing/restrained energy minimization
Expression systems:
- Escherichia coli
- Escherichia coli BL21(DE3)
