› 1t6u

X-ray diffraction
1.3Å resolution

Nickel Superoxide Dismutase (NiSOD) Native 1.30 A Structure

Released:
DOI: 10.2210/pdb1t6u/pdb
PDB entry 1t6u coloured by chain, front view.
PDB entry 1t6u coloured by chain, side view.
PDB entry 1t6u coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Nickel superoxide dismutase structure and mechanism.
Barondeau DP, Kassmann CJ, Bruns CK, Tainer JA, Getzoff ED
Biochemistry 43 8038-47 (2004)
PMID: 15209499

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Ni] Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 117 amino acids
Theoretical weight: 13.24 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: P80735 (Residues: 15-131; Coverage: 89%)
Gene names: 2SC7G11.16c, SCO5254, sod1, sodN
Sequence domains: Nickel-containing superoxide dismutase
Structure domains: Nickel-containing superoxide dismutase

Ligands and Environments

1 bound ligand:
Ligand NI 12 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 64.147Å b: 111.68Å c: 106.834Å
α: 90° β: 94.51° γ: 90°
R-values:
R R work R free
0.158 0.158 0.201
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Free radicals, metals and antioxidants in oxidative stress-induced cancer.
Valko et al. (2006)
23 more

12 mentions without citation

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
11 more