› 1szw

X-ray diffraction
2Å resolution

Crystal structure of E. coli tRNA pseudouridine synthase TruD

Released:
DOI: 10.2210/pdb1szw/pdb
PDB entry 1szw coloured by chain, front view.
PDB entry 1szw coloured by chain, side view.
PDB entry 1szw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold.
Ericsson UB, Nordlund P, Hallberg BM
FEBS Lett 565 59-64 (2004)
PMID: 15135053

Function and Biology Details

Reaction catalysed: 
tRNA uridine(13) = tRNA pseudouridine(13)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase D Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 42.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57261 (Residues: 1-349; Coverage: 100%)
Gene names: JW2715, b2745, truD, ygbO
Sequence domains: tRNA pseudouridine synthase D (TruD)
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 63.18Å b: 108.11Å c: 112.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Pseudouridine synthases.
Hamma et al. (2006)
7 more

1 mention without citation

Autonomous aggregation suppression by acidic residues explains why chaperones favour basic residues.
Houben et al. (2020)