› 1sxg

X-ray diffraction
2.75Å resolution

Structural studies on the apo transcription factor form B. megaterium

Released:
DOI: 10.2210/pdb1sxg/pdb
PDB entry 1sxg coloured by chain, front view.
PDB entry 1sxg coloured by chain, side view.
PDB entry 1sxg coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.
Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG
Cell 118 731-41 (2004)
PMID: 15369672

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catabolite control protein A Chains: A, B, D, F, I, P
Molecule details ›
Chains: A, B, D, F, I, P
Length: 280 amino acids
Theoretical weight: 30.99 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46828 (Residues: 53-332; Coverage: 84%)
Gene name: ccpA
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand 171 2 x 171
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 375.39Å b: 60.14Å c: 75.65Å
α: 90° β: 95.59° γ: 90°
R-values:
R R work R free
0.223 0.214 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Carbon catabolite repression in bacteria: many ways to make the most out of nutrients.
Görke et al. (2008)
18 more

1 mention without citation

Structure of the apo form of the catabolite control protein A (CcpA) from Bacillus megaterium with a DNA-binding domain.
Singh et al. (2007)