› 1ssm

X-ray diffraction
2.15Å resolution

Serine Acetyltransferase- Apoenzyme (truncated)

Released:
DOI: 10.2210/pdb1ssm/pdb
PDB entry 1ssm coloured by chain, front view.
PDB entry 1ssm coloured by chain, side view.
PDB entry 1ssm coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Structure of serine acetyltransferase in complexes with CoA and its cysteine feedback inhibitor.
Olsen LR, Huang B, Vetting MW, Roderick SL
Biochemistry 43 6013-9 (2004)
PMID: 15147185

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine acetyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 242 amino acids
Theoretical weight: 26.65 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43886 (Residues: 1-242; Coverage: 91%)
Gene names: HI_0606, cysE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P21212
Unit cell:
a: 107.1Å b: 126.6Å c: 107.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.278
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Selenium uptake, translocation, assimilation and metabolic fate in plants.
Sors et al. (2005)
10 more

5 mentions without citation

Combatting antimicrobial resistance via the cysteine biosynthesis pathway in bacterial pathogens.
Hicks et al. (2022)
4 more