› 1srr

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A PHOSPHATASE RESISTANT MUTANT OF SPORULATION RESPONSE REGULATOR SPO0F FROM BACILLUS SUBTILIS

Released:
DOI: 10.2210/pdb1srr/pdb
PDB entry 1srr coloured by chain, front view.
PDB entry 1srr coloured by chain, side view.
PDB entry 1srr coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of a phosphatase-resistant mutant of sporulation response regulator Spo0F from Bacillus subtilis.
Zapf J, Whiteley JM, Hoch JA, Xuong NH, Varughese KI
Structure 4 679-90 (1996)
PMID: 8805550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sporulation initiation phosphotransferase F Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 124 amino acids
Theoretical weight: 14.17 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06628 (Residues: 1-124; Coverage: 100%)
Gene names: BSU37130, spo0F
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P43212
Unit cell:
a: 101.99Å b: 101.99Å c: 82.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.278
Expression system: Escherichia coli

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Citations

5 review citations

Two-component signal transduction.
Stock et al. (2000)
4 more

9 mentions without citation

Chemical shift prediction for protein structure calculation and quality assessment using an optimally parameterized force field.
Nielsen et al. (2012)
8 more