› 1sre

X-ray diffraction
1.78Å resolution

CRYSTALLOGRAPHIC AND THERMODYNAMIC COMPARISON OF NATURAL AND SYNTHETIC LIGANDS BOUND TO STREPTAVIDIN

Released:
DOI: 10.2210/pdb1sre/pdb
PDB entry 1sre coloured by chain, front view.
PDB entry 1sre coloured by chain, side view.
PDB entry 1sre coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Structure-Based Design of Synthetic Azobenzene Ligands for Streptavidin
Weber PC, Pantoliano MW, Simons DM, Salemme FR
J. Am. Chem. Soc. 116 2717-2724 (1994)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 12.74 KDa
Source organism: Streptomyces avidinii
Expression system: Not provided
UniProt:
  • Canonical: P22629 (Residues: 37-157; Coverage: 76%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand HAB 2 x HAB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 95.2Å b: 106.5Å c: 47.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 not available not available
Expression system: Not provided

Quick links

Citations

6 mentions without citation

LIGSITEcsc: predicting ligand binding sites using the Connolly surface and degree of conservation.
Huang et al. (2006)
5 more