› 1son

X-ray diffraction
2.55Å resolution

ADENYLOSUCCINATE SYNTHETASE IN COMPLEX WITH THE NATURAL FEEDBACK INHIBITOR AMP

Released:
DOI: 10.2210/pdb1son/pdb
PDB entry 1son coloured by chain, front view.
PDB entry 1son coloured by chain, side view.
PDB entry 1son coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The mode of action and the structure of a herbicide in complex with its target: binding of activated hydantocidin to the feedback regulation site of adenylosuccinate synthetase.
Fonné-Pfister R, Chemla P, Ward E, Girardet M, Kreuz KE, Honzatko RB, Fromm HJ, Schär HP, Grütter MG, Cowan-Jacob SW
Proc Natl Acad Sci U S A 93 9431-6 (1996)
PMID: 8790347

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 47.27 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7D4 (Residues: 2-432; Coverage: 100%)
Gene names: JW4135, adeK, b4177, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AMP 1 x AMP
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 70.5Å b: 70.5Å c: 201.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 not available

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Citations

6 review citations

Pyrimidine and purine biosynthesis and degradation in plants.
Zrenner et al. (2006)
5 more

1 mention without citation

On the molecular discrimination between adenine and guanine by proteins.
Nobeli et al. (2001)