› 1sj9

X-ray diffraction
2.5Å resolution

Crystal structure of the uridine phosphorylase from Salmonella typhimurium at 2.5A resolution

Released:
DOI: 10.2210/pdb1sj9/pdb
PDB entry 1sj9 coloured by chain, front view.
PDB entry 1sj9 coloured by chain, side view.
PDB entry 1sj9 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state.
Dontsova MV, Gabdoulkhakov AG, Molchan OK, Lashkov AA, Garber MB, Mironov AS, Zhukhlistova NE, Morgunova EY, Voelter W, Betzel C, Zhang Y, Ealick SE, Mikhailov AM
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 337-40 (2005)
PMID: 16511035

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 253 amino acids
Theoretical weight: 27.17 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1F6 (Residues: 1-253; Coverage: 100%)
Gene names: STM3968, STMD1.21, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P61
Unit cell:
a: 91.37Å b: 91.37Å c: 266.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy.
Roosild et al. (2009)
4 more

6 mentions without citation

Recent applications of computational methods to allosteric drug discovery.
Govindaraj et al. (2022)
5 more