› 1shk

X-ray diffraction
1.9Å resolution

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI

Released:
DOI: 10.2210/pdb1shk/pdb
PDB entry 1shk coloured by chain, front view.
PDB entry 1shk coloured by chain, side view.
PDB entry 1shk coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi.
Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ
Acta Crystallogr D Biol Crystallogr 53 612-4 (1997)
PMID: 15299895

Function and Biology Details

Reaction catalysed: 
ATP + shikimate = ADP + shikimate 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-145505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 18.98 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P10880 (Residues: 1-173; Coverage: 100%)
Gene names: aroL, aroM
Sequence domains: Shikimate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P41212
Unit cell:
a: 108.5Å b: 108.5Å c: 92.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.176 0.222
Expression system: Escherichia coli

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Citations

1 review citation

The shikimate pathway: review of amino acid sequence, function and three-dimensional structures of the enzymes.
Mir et al. (2015)
2 other articles

5 mentions without citation

Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme.
Wang et al. (2002)
4 more