› 1s4v

X-ray diffraction
2Å resolution

The 2.0 A crystal structure of the KDEL-tailed cysteine endopeptidase functioning in programmed cell death of Ricinus communis endosperm

Released:
DOI: 10.2210/pdb1s4v/pdb
PDB entry 1s4v coloured by chain, front view.
PDB entry 1s4v coloured by chain, side view.
PDB entry 1s4v coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
The 2.0 A crystal structure and substrate specificity of the KDEL-tailed cysteine endopeptidase functioning in programmed cell death of Ricinus communis endosperm.
Than ME, Helm M, Simpson DJ, Lottspeich F, Huber R, Gietl C
J Mol Biol 336 1103-16 (2004)
PMID: 15037072

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130286 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vignain Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 24.6 KDa
Source organism: Ricinus communis
UniProt:
  • Canonical: O65039 (Residues: 125-353; Coverage: 67%)
Gene name: CYSEP
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases
DVA-LEU-LYS-0QE peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 392 Da
Source organism: Ricinus communis

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand DVA 2 x DVA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 36.448Å b: 68.162Å c: 163.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.226

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Citations

5 review citations

A cut above the rest: the regulatory function of plant proteases.
Schaller A. (2004)
4 more

9 mentions without citation

An effector from the Huanglongbing-associated pathogen targets citrus proteases.
Clark et al. (2018)
8 more